Construct: shRNA TRCN0000367029

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001164663.1-1012s21c1
DNA Barcode:
TGCGTTCCGAGAACGACTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Soga1 (320706)
Gene Description:
suppressor of glucose, autophagy associated 1
Transcript:
RefSeq NM_001164663.1 (CURRENT)
Match location:
Position 1012 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367029)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCGTTCCGAGAACGACTATC
Hairpin Sequence:
5'-CCGG-TGCGTTCCGAGAACGACTATC-CTCGAG-GATAGTCGTTCTCGGAACGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCGTTCCGAGAACGACTATCCTCGAGGATAGTCGTTCTCGGAACGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCGTTCCGAGAACGACTATCCTCGAGGATAGTCGTTCTCGGAACGCA-3'

Other clones with same target sequence:

(none)