Construct: shRNA TRCN0000367459

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006240.2-2314s21c1
DNA Barcode:
CAATAAGCTTGCCAACATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPEF1 (5475)
Gene Description:
protein phosphatase with EF-hand domain 1
Transcript:
RefSeq NM_006240.2 (CURRENT)
Match location:
Position 2314 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000367459)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATAAGCTTGCCAACATAAT
Hairpin Sequence:
5'-CCGG-CAATAAGCTTGCCAACATAAT-CTCGAG-ATTATGTTGGCAAGCTTATTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATAAGCTTGCCAACATAATCTCGAGATTATGTTGGCAAGCTTATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATAAGCTTGCCAACATAATCTCGAGATTATGTTGGCAAGCTTATTG-3'

Other clones with same target sequence:

(none)