Construct: shRNA TRCN0000368714

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007939.2-2120s21c1
DNA Barcode:
CAGCCATCATGGGCCAATTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Epha8 (13842)
Gene Description:
Eph receptor A8
Transcript:
RefSeq NM_007939.2 (CURRENT)
Match location:
Position 2120 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000368714)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCCATCATGGGCCAATTTG
Hairpin Sequence:
5'-CCGG-CAGCCATCATGGGCCAATTTG-CTCGAG-CAAATTGGCCCATGATGGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCCATCATGGGCCAATTTGCTCGAGCAAATTGGCCCATGATGGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCCATCATGGGCCAATTTGCTCGAGCAAATTGGCCCATGATGGCTG-3'

Other clones with same target sequence:

(none)