Construct: shRNA TRCN0000371416

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181704.1-295s21c1
DNA Barcode:
GCACAGCTCTGTGATTCATCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BAGE4 (85317)
Gene Description:
BAGE family member 4
Transcript:
RefSeq NM_181704.1 (CURRENT)
Match location:
Position 295 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371416)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGCTCTGTGATTCATCT
Hairpin Sequence:
5'-CCGG-GCACAGCTCTGTGATTCATCT-CTCGAG-AGATGAATCACAGAGCTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGCTCTGTGATTCATCTCTCGAGAGATGAATCACAGAGCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGCTCTGTGATTCATCTCTCGAGAGATGAATCACAGAGCTGTGC-3'

Other clones with same target sequence:

(none)