Transcript: Human NM_181704.1

Homo sapiens BAGE family member 4 (BAGE4), mRNA.

Source:
NCBI, updated 2019-09-21
Taxon:
Homo sapiens (human)
Gene:
BAGE4 (85317)
Length:
1840
CDS:
189..308

Additional Resources:

NCBI RefSeq record:
NM_181704.1
NBCI Gene record:
BAGE4 (85317)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_181704.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000168195 CCAGAAAGATCGAAGGAAGAT pLKO.1 547 3UTR 100% 4.950 2.970 N BAGE4 n/a
2 TRCN0000173051 CGAGGCAAGTCAAAGCTGAAA pLKO.1 1612 3UTR 100% 4.950 2.970 N BAGE4 n/a
3 TRCN0000371413 CTTCTGAAGTGAAGCATATTT pLKO_005 904 3UTR 100% 15.000 7.500 Y BAGE3 n/a
4 TRCN0000371417 TATAGCGGTTACTCCATTAAA pLKO_005 660 3UTR 100% 15.000 7.500 Y BAGE4 n/a
5 TRCN0000371414 ACTTTGGAGCCACTATCAAAT pLKO_005 413 3UTR 100% 13.200 6.600 Y BAGE3 n/a
6 TRCN0000168224 CCTGAGGAACAGTTGGTTATA pLKO.1 1143 3UTR 100% 13.200 6.600 Y BAGE2 n/a
7 TRCN0000168341 GCATGGGTAAACCAGCTATTA pLKO.1 1484 3UTR 100% 13.200 6.600 Y BAGE2 n/a
8 TRCN0000371415 GATATAGCGGTTACTCCATTA pLKO_005 658 3UTR 100% 10.800 5.400 Y BAGE3 n/a
9 TRCN0000377661 GGCCAAGTGCGATGTGCATTT pLKO_005 385 3UTR 100% 10.800 5.400 Y BAGE4 n/a
10 TRCN0000168024 CGTGTGACAAAGGGTATCATA pLKO.1 767 3UTR 100% 5.625 2.813 Y BAGE2 n/a
11 TRCN0000167620 GAGGAACAGTTGGTTATAGAA pLKO.1 1146 3UTR 100% 5.625 2.813 Y BAGE5 n/a
12 TRCN0000168695 GCAAACAATCGGGAGAAGATA pLKO.1 725 3UTR 100% 5.625 2.813 Y BAGE4 n/a
13 TRCN0000172937 GCACTTTGGAGCCACTATCAA pLKO.1 411 3UTR 100% 5.625 2.813 Y BAGE2 n/a
14 TRCN0000167349 CTTTCAGGATTTCAGTCACAT pLKO.1 492 3UTR 100% 4.950 2.475 Y BAGE2 n/a
15 TRCN0000168607 GATCGAAGGAAGATGCAAACT pLKO.1 554 3UTR 100% 4.950 2.475 Y BAGE2 n/a
16 TRCN0000168340 GCAAGATGCTAGTGTGTGATA pLKO.1 746 3UTR 100% 4.950 2.475 Y BAGE4 n/a
17 TRCN0000371416 GCACAGCTCTGTGATTCATCT pLKO_005 295 CDS 100% 4.950 2.475 Y BAGE4 n/a
18 TRCN0000168767 GCAGTTGTTAGAGGAACCTAA pLKO.1 1013 3UTR 100% 4.950 2.475 Y BAGE4 n/a
19 TRCN0000168639 GTGTGTGATACGTGTGACAAA pLKO.1 757 3UTR 100% 4.950 2.475 Y BAGE5 n/a
20 TRCN0000166864 CAGATGTATCATTATCCTTGT pLKO.1 454 3UTR 100% 4.050 2.025 Y BAGE3 n/a
21 TRCN0000167251 CAGATTTCTAATGAGGCTGAT pLKO.1 864 3UTR 100% 4.050 2.025 Y BAGE4 n/a
22 TRCN0000172796 GCTGGAAACATCATGCCAACA pLKO.1 1434 3UTR 100% 4.050 2.025 Y BAGE5 n/a
23 TRCN0000168727 GTTTCATACCAAGGAGGCAAA pLKO.1 1266 3UTR 100% 4.050 2.025 Y BAGE5 n/a
24 TRCN0000173000 CCAGAACACATTGACCAAGCT pLKO.1 526 3UTR 100% 2.640 1.320 Y BAGE2 n/a
25 TRCN0000168402 GAAAGATCGAAGGAAGATGCA pLKO.1 550 3UTR 100% 2.640 1.320 Y BAGE3 n/a
26 TRCN0000167726 GAAATGCAAATGAATACCGAA pLKO.1 834 3UTR 100% 2.640 1.320 Y BAGE5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_181704.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00148 pDONR223 100% 88.3% 86% None (many diffs) n/a
2 ccsbBroad304_00148 pLX_304 0% 88.3% 86% V5 (many diffs) n/a
3 TRCN0000472238 AGTATTTGCGGTAGTCCAGACGAC pLX_317 100% 88.3% 86% V5 (many diffs) n/a
4 ccsbBroadEn_09255 pDONR223 100% 33.9% 33.9% None (many diffs) n/a
5 ccsbBroad304_09255 pLX_304 0% 33.9% 33.9% V5 (many diffs) n/a
6 TRCN0000468075 ATACTCGCAATGAAACTCCGACGA pLX_317 100% 33.9% 33.9% V5 (many diffs) n/a
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