Construct: shRNA TRCN0000371549

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000716.3-787s21c1
DNA Barcode:
GTGAAGACAGGTACTACTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C4BPB (725)
Gene Description:
complement component 4 binding protein beta
Transcript:
RefSeq NM_000716.3 (CURRENT)
Match location:
Position 787 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371549)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAAGACAGGTACTACTTAG
Hairpin Sequence:
5'-CCGG-GTGAAGACAGGTACTACTTAG-CTCGAG-CTAAGTAGTACCTGTCTTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAAGACAGGTACTACTTAGCTCGAGCTAAGTAGTACCTGTCTTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAAGACAGGTACTACTTAGCTCGAGCTAAGTAGTACCTGTCTTCAC-3'

Other clones with same target sequence:

(none)