Construct: shRNA TRCN0000371959

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006419.2-399s21c1
DNA Barcode:
GATTCCCTGATGCTGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CXCL13 (10563)
Gene Description:
C-X-C motif chemokine ligand 13
Transcript:
RefSeq NM_006419.2 (CURRENT)
Match location:
Position 399 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000371959)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTCCCTGATGCTGATATTT
Hairpin Sequence:
5'-CCGG-GATTCCCTGATGCTGATATTT-CTCGAG-AAATATCAGCATCAGGGAATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTCCCTGATGCTGATATTTCTCGAGAAATATCAGCATCAGGGAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTCCCTGATGCTGATATTTCTCGAGAAATATCAGCATCAGGGAATC-3'

Other clones with same target sequence:

(none)