Construct: shRNA TRCN0000373508

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000488.3-802s21c1
DNA Barcode:
GCCGAATCACCGATGTCATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SERPINC1 (462)
Gene Description:
serpin family C member 1
Transcript:
RefSeq NM_000488.3 (CURRENT)
Match location:
Position 802 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000373508)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAATCACCGATGTCATTC
Hairpin Sequence:
5'-CCGG-GCCGAATCACCGATGTCATTC-CTCGAG-GAATGACATCGGTGATTCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAATCACCGATGTCATTCCTCGAGGAATGACATCGGTGATTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAATCACCGATGTCATTCCTCGAGGAATGACATCGGTGATTCGGC-3'

Other clones with same target sequence:

(none)