Construct: shRNA TRCN0000374143

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009584.4-428s21c1
DNA Barcode:
ACAGATCAAAGCAGCTCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnajc2 (22791)
Gene Description:
DnaJ heat shock protein family (Hsp40) member C2
Transcript:
RefSeq NM_009584.4 (CURRENT)
Match location:
Position 428 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000374143)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGATCAAAGCAGCTCATAA
Hairpin Sequence:
5'-CCGG-ACAGATCAAAGCAGCTCATAA-CTCGAG-TTATGAGCTGCTTTGATCTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGATCAAAGCAGCTCATAACTCGAGTTATGAGCTGCTTTGATCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGATCAAAGCAGCTCATAACTCGAGTTATGAGCTGCTTTGATCTGT-3'

Other clones with same target sequence:

  1. TRCN0000254055