Construct: shRNA TRCN0000374513

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015775.2-876s21c1
DNA Barcode:
GGCAACGTTGACCTCTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmprss2 (50528)
Gene Description:
transmembrane protease, serine 2
Transcript:
RefSeq NM_015775.2 (CURRENT)
Match location:
Position 876 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000374513)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCAACGTTGACCTCTATAAA
Hairpin Sequence:
5'-CCGG-GGCAACGTTGACCTCTATAAA-CTCGAG-TTTATAGAGGTCAACGTTGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCAACGTTGACCTCTATAAACTCGAGTTTATAGAGGTCAACGTTGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCAACGTTGACCTCTATAAACTCGAGTTTATAGAGGTCAACGTTGCC-3'

Other clones with same target sequence:

(none)