Construct: shRNA TRCN0000374705

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172974.2-181s21c1
DNA Barcode:
ACCTCTTGGAGCAGTTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cops7b (26895)
Gene Description:
COP9 signalosome subunit 7B
Transcript:
RefSeq NM_172974.2 (CURRENT)
Match location:
Position 181 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000374705)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTCTTGGAGCAGTTCATTT
Hairpin Sequence:
5'-CCGG-ACCTCTTGGAGCAGTTCATTT-CTCGAG-AAATGAACTGCTCCAAGAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTCTTGGAGCAGTTCATTTCTCGAGAAATGAACTGCTCCAAGAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTCTTGGAGCAGTTCATTTCTCGAGAAATGAACTGCTCCAAGAGGT-3'

Other clones with same target sequence:

(none)