Construct: shRNA TRCN0000374945

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178610.4-1517s21c1
DNA Barcode:
CCATTGGTTGGTCTTCATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krr1 (52705)
Gene Description:
KRR1, small subunit (SSU) processome component, homolog (yeast)
Transcript:
RefSeq NM_178610.4 (CURRENT)
Match location:
Position 1517 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000374945)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGGTTGGTCTTCATAAT
Hairpin Sequence:
5'-CCGG-CCATTGGTTGGTCTTCATAAT-CTCGAG-ATTATGAAGACCAACCAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGGTTGGTCTTCATAATCTCGAGATTATGAAGACCAACCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGGTTGGTCTTCATAATCTCGAGATTATGAAGACCAACCAATGG-3'

Other clones with same target sequence:

(none)