Construct: shRNA TRCN0000375001

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153125.2-3885s21c1
DNA Barcode:
ATGCCGAGAACCACCGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec16a (227648)
Gene Description:
SEC16 homolog A, endoplasmic reticulum export factor
Transcript:
RefSeq NM_153125.2 (CURRENT)
Match location:
Position 3885 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375001)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCCGAGAACCACCGCTATT
Hairpin Sequence:
5'-CCGG-ATGCCGAGAACCACCGCTATT-CTCGAG-AATAGCGGTGGTTCTCGGCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCCGAGAACCACCGCTATTCTCGAGAATAGCGGTGGTTCTCGGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCCGAGAACCACCGCTATTCTCGAGAATAGCGGTGGTTCTCGGCAT-3'

Other clones with same target sequence:

(none)