Construct: shRNA TRCN0000375479

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144858.2-1307s21c1
DNA Barcode:
CCGCCAAGTTCCAACAGATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dus3l (224907)
Gene Description:
dihydrouridine synthase 3-like (S. cerevisiae)
Transcript:
RefSeq NM_144858.2 (CURRENT)
Match location:
Position 1307 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375479)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCCAAGTTCCAACAGATTG
Hairpin Sequence:
5'-CCGG-CCGCCAAGTTCCAACAGATTG-CTCGAG-CAATCTGTTGGAACTTGGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCCAAGTTCCAACAGATTGCTCGAGCAATCTGTTGGAACTTGGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCCAAGTTCCAACAGATTGCTCGAGCAATCTGTTGGAACTTGGCGG-3'

Other clones with same target sequence:

(none)