Construct: shRNA TRCN0000375722

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173440.2-2230s21c1
DNA Barcode:
CAGTTGCTGCTCGGCCATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nrip1 (268903)
Gene Description:
nuclear receptor interacting protein 1
Transcript:
RefSeq NM_173440.2 (CURRENT)
Match location:
Position 2230 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375722)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTTGCTGCTCGGCCATAAA
Hairpin Sequence:
5'-CCGG-CAGTTGCTGCTCGGCCATAAA-CTCGAG-TTTATGGCCGAGCAGCAACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTTGCTGCTCGGCCATAAACTCGAGTTTATGGCCGAGCAGCAACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTTGCTGCTCGGCCATAAACTCGAGTTTATGGCCGAGCAGCAACTG-3'

Other clones with same target sequence:

(none)