Construct: shRNA TRCN0000375931

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011249.2-1771s21c1
DNA Barcode:
GAGTTTGGCTTGGACTAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbl1 (19650)
Gene Description:
retinoblastoma-like 1 (p107)
Transcript:
RefSeq NM_011249.2 (CURRENT)
Match location:
Position 1771 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375931)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTTGGCTTGGACTAATAA
Hairpin Sequence:
5'-CCGG-GAGTTTGGCTTGGACTAATAA-CTCGAG-TTATTAGTCCAAGCCAAACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTTGGCTTGGACTAATAACTCGAGTTATTAGTCCAAGCCAAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTTGGCTTGGACTAATAACTCGAGTTATTAGTCCAAGCCAAACTC-3'

Other clones with same target sequence:

(none)