Construct: shRNA TRCN0000375960

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008615.2-2159s21c1
DNA Barcode:
CTGATATGTAGGAAGCTAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Me1 (17436)
Gene Description:
malic enzyme 1, NADP(+)-dependent, cytosolic
Transcript:
RefSeq NM_008615.2 (CURRENT)
Match location:
Position 2159 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375960)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGATATGTAGGAAGCTAATG
Hairpin Sequence:
5'-CCGG-CTGATATGTAGGAAGCTAATG-CTCGAG-CATTAGCTTCCTACATATCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGATATGTAGGAAGCTAATGCTCGAGCATTAGCTTCCTACATATCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGATATGTAGGAAGCTAATGCTCGAGCATTAGCTTCCTACATATCAG-3'

Other clones with same target sequence:

(none)