Construct: shRNA TRCN0000376093

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019791.2-1525s21c1
DNA Barcode:
ACTTACGACCCTCACCTAATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Maged1 (94275)
Gene Description:
melanoma antigen, family D, 1
Transcript:
RefSeq NM_019791.2 (CURRENT)
Match location:
Position 1525 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376093)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTACGACCCTCACCTAATC
Hairpin Sequence:
5'-CCGG-ACTTACGACCCTCACCTAATC-CTCGAG-GATTAGGTGAGGGTCGTAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTACGACCCTCACCTAATCCTCGAGGATTAGGTGAGGGTCGTAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTACGACCCTCACCTAATCCTCGAGGATTAGGTGAGGGTCGTAAGT-3'

Other clones with same target sequence:

(none)