Construct: shRNA TRCN0000376308

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001164565.1-1486s21c1
DNA Barcode:
TCTGATATGTTCCTGATAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acnat1 (230161)
Gene Description:
acyl-coenzyme A amino acid N-acyltransferase 1
Transcript:
RefSeq NM_001164565.1 (CURRENT)
Match location:
Position 1486 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376308)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGATATGTTCCTGATAAAT
Hairpin Sequence:
5'-CCGG-TCTGATATGTTCCTGATAAAT-CTCGAG-ATTTATCAGGAACATATCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGATATGTTCCTGATAAATCTCGAGATTTATCAGGAACATATCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGATATGTTCCTGATAAATCTCGAGATTTATCAGGAACATATCAGA-3'

Other clones with same target sequence:

(none)