Construct: shRNA TRCN0000376545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004923.3-1854s21c1
DNA Barcode:
GCATGGCCTGAGCATTGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TESMIN (9633)
Gene Description:
testis expressed metallothionein like protein
Transcript:
RefSeq NM_004923.3 (CURRENT)
Match location:
Position 1854 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGGCCTGAGCATTGTTTG
Hairpin Sequence:
5'-CCGG-GCATGGCCTGAGCATTGTTTG-CTCGAG-CAAACAATGCTCAGGCCATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGGCCTGAGCATTGTTTGCTCGAGCAAACAATGCTCAGGCCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGGCCTGAGCATTGTTTGCTCGAGCAAACAATGCTCAGGCCATGC-3'

Other clones with same target sequence:

(none)