Construct: shRNA TRCN0000376979

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026529.4-668s21c1
DNA Barcode:
ACTTCTTATCTTCTCTATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2700062C07Rik (68046)
Gene Description:
RIKEN cDNA 2700062C07 gene
Transcript:
RefSeq NM_026529.4 (CURRENT)
Match location:
Position 668 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376979)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTCTTATCTTCTCTATGAA
Hairpin Sequence:
5'-CCGG-ACTTCTTATCTTCTCTATGAA-CTCGAG-TTCATAGAGAAGATAAGAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTCTTATCTTCTCTATGAACTCGAGTTCATAGAGAAGATAAGAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTCTTATCTTCTCTATGAACTCGAGTTCATAGAGAAGATAAGAAGT-3'

Other clones with same target sequence:

(none)