Construct: shRNA TRCN0000377192

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029497.1-7142s21c1
DNA Barcode:
TTGGTCATGGATTGGTATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Urb1 (207932)
Gene Description:
URB1 ribosome biogenesis 1 homolog (S. cerevisiae)
Transcript:
RefSeq NM_029497.1 (CURRENT)
Match location:
Position 7142 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377192)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGTCATGGATTGGTATTAA
Hairpin Sequence:
5'-CCGG-TTGGTCATGGATTGGTATTAA-CTCGAG-TTAATACCAATCCATGACCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGTCATGGATTGGTATTAACTCGAGTTAATACCAATCCATGACCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGTCATGGATTGGTATTAACTCGAGTTAATACCAATCCATGACCAA-3'

Other clones with same target sequence:

(none)