Construct: shRNA TRCN0000377723

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018363.3-1150s21c1
DNA Barcode:
AGCCTTTAGGATAGCTATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNLS (55328)
Gene Description:
renalase, FAD dependent amine oxidase
Transcript:
RefSeq NM_018363.3 (CURRENT)
Match location:
Position 1150 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377723)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCTTTAGGATAGCTATTAT
Hairpin Sequence:
5'-CCGG-AGCCTTTAGGATAGCTATTAT-CTCGAG-ATAATAGCTATCCTAAAGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCTTTAGGATAGCTATTATCTCGAGATAATAGCTATCCTAAAGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCTTTAGGATAGCTATTATCTCGAGATAATAGCTATCCTAAAGGCT-3'

Other clones with same target sequence:

(none)