Construct: shRNA TRCN0000378856

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002527.4-568s21c1
DNA Barcode:
AGTCATCGGCCATCGACATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NTF3 (4908)
Gene Description:
neurotrophin 3
Transcript:
RefSeq NM_002527.4 (CURRENT)
Match location:
Position 568 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000378856)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCATCGGCCATCGACATTC
Hairpin Sequence:
5'-CCGG-AGTCATCGGCCATCGACATTC-CTCGAG-GAATGTCGATGGCCGATGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCATCGGCCATCGACATTCCTCGAGGAATGTCGATGGCCGATGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCATCGGCCATCGACATTCCTCGAGGAATGTCGATGGCCGATGACT-3'

Other clones with same target sequence:

(none)