Construct: shRNA TRCN0000379291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146153.3-259s21c1
DNA Barcode:
GCCGATCTGTCTCTCGTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Thrap3 (230753)
Gene Description:
thyroid hormone receptor associated protein 3
Transcript:
RefSeq NM_146153.3 (CURRENT)
Match location:
Position 259 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGATCTGTCTCTCGTTCAA
Hairpin Sequence:
5'-CCGG-GCCGATCTGTCTCTCGTTCAA-CTCGAG-TTGAACGAGAGACAGATCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGATCTGTCTCTCGTTCAACTCGAGTTGAACGAGAGACAGATCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGATCTGTCTCTCGTTCAACTCGAGTTGAACGAGAGACAGATCGGC-3'

Other clones with same target sequence:

(none)