Construct: shRNA TRCN0000379394

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023420.2-1004s21c1
DNA Barcode:
ATGAGCTTCAGAGGGATAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col4a3bp (68018)
Gene Description:
collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
Transcript:
RefSeq NM_023420.2 (CURRENT)
Match location:
Position 1004 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379394)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGAGCTTCAGAGGGATAAAG
Hairpin Sequence:
5'-CCGG-ATGAGCTTCAGAGGGATAAAG-CTCGAG-CTTTATCCCTCTGAAGCTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGAGCTTCAGAGGGATAAAGCTCGAGCTTTATCCCTCTGAAGCTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGAGCTTCAGAGGGATAAAGCTCGAGCTTTATCCCTCTGAAGCTCAT-3'

Other clones with same target sequence:

(none)