Construct: shRNA TRCN0000379766

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030725.4-1153s21c1
DNA Barcode:
GGAACGAGATGATCATGTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Syt13 (80976)
Gene Description:
synaptotagmin XIII
Transcript:
RefSeq NM_030725.4 (CURRENT)
Match location:
Position 1153 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000379766)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAACGAGATGATCATGTTTG
Hairpin Sequence:
5'-CCGG-GGAACGAGATGATCATGTTTG-CTCGAG-CAAACATGATCATCTCGTTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAACGAGATGATCATGTTTGCTCGAGCAAACATGATCATCTCGTTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAACGAGATGATCATGTTTGCTCGAGCAAACATGATCATCTCGTTCC-3'

Other clones with same target sequence:

(none)