Construct: shRNA TRCN0000380199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019788.3-366s21c1
DNA Barcode:
ATACACTGGAACAAGAGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bloc1s6 (18457)
Gene Description:
biogenesis of lysosomal organelles complex-1, subunit 6, pallidin
Transcript:
RefSeq NM_019788.3 (CURRENT)
Match location:
Position 366 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATACACTGGAACAAGAGATTT
Hairpin Sequence:
5'-CCGG-ATACACTGGAACAAGAGATTT-CTCGAG-AAATCTCTTGTTCCAGTGTAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATACACTGGAACAAGAGATTTCTCGAGAAATCTCTTGTTCCAGTGTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATACACTGGAACAAGAGATTTCTCGAGAAATCTCTTGTTCCAGTGTAT-3'

Other clones with same target sequence:

(none)