Construct: shRNA TRCN0000380714

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016578.3-3944s21c1
DNA Barcode:
GCCGAAGCACAGACGAGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RSF1 (51773)
Gene Description:
remodeling and spacing factor 1
Transcript:
RefSeq NM_016578.3 (CURRENT)
Match location:
Position 3944 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380714)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAAGCACAGACGAGTATT
Hairpin Sequence:
5'-CCGG-GCCGAAGCACAGACGAGTATT-CTCGAG-AATACTCGTCTGTGCTTCGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAAGCACAGACGAGTATTCTCGAGAATACTCGTCTGTGCTTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAAGCACAGACGAGTATTCTCGAGAATACTCGTCTGTGCTTCGGC-3'

Other clones with same target sequence:

(none)