Construct: shRNA TRCN0000380886

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018804.3-1376s21c1
DNA Barcode:
CACCGACCTTCTGCCTGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Syt11 (229521)
Gene Description:
synaptotagmin XI
Transcript:
RefSeq NM_018804.3 (CURRENT)
Match location:
Position 1376 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000380886)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCGACCTTCTGCCTGATAT
Hairpin Sequence:
5'-CCGG-CACCGACCTTCTGCCTGATAT-CTCGAG-ATATCAGGCAGAAGGTCGGTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCGACCTTCTGCCTGATATCTCGAGATATCAGGCAGAAGGTCGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCGACCTTCTGCCTGATATCTCGAGATATCAGGCAGAAGGTCGGTG-3'

Other clones with same target sequence:

(none)