Construct: shRNA TRCN0000382021

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175414.4-442s21c1
DNA Barcode:
TCATCGTGCTGTTGATCATCC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tspan9 (109246)
Gene Description:
tetraspanin 9
Transcript:
RefSeq NM_175414.4 (CURRENT)
Match location:
Position 442 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000382021)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATCGTGCTGTTGATCATCC
Hairpin Sequence:
5'-CCGG-TCATCGTGCTGTTGATCATCC-CTCGAG-GGATGATCAACAGCACGATGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATCGTGCTGTTGATCATCCCTCGAGGGATGATCAACAGCACGATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATCGTGCTGTTGATCATCCCTCGAGGGATGATCAACAGCACGATGA-3'

Other clones with same target sequence:

(none)