Construct: shRNA TRCN0000382099

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004615.3-192s21c1
DNA Barcode:
ACTTACTCTGGGCACCTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TSPAN7 (7102)
Gene Description:
tetraspanin 7
Transcript:
RefSeq NM_004615.3 (CURRENT)
Match location:
Position 192 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000382099)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTACTCTGGGCACCTATAT
Hairpin Sequence:
5'-CCGG-ACTTACTCTGGGCACCTATAT-CTCGAG-ATATAGGTGCCCAGAGTAAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTACTCTGGGCACCTATATCTCGAGATATAGGTGCCCAGAGTAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTACTCTGGGCACCTATATCTCGAGATATAGGTGCCCAGAGTAAGT-3'

Other clones with same target sequence:

(none)