Construct: shRNA TRCN0000382428

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025783.3-1107s21c1
DNA Barcode:
GAATGATGAGGACTCTATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chmp3 (66700)
Gene Description:
charged multivesicular body protein 3
Transcript:
RefSeq NM_025783.3 (CURRENT)
Match location:
Position 1107 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000382428)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGATGAGGACTCTATATT
Hairpin Sequence:
5'-CCGG-GAATGATGAGGACTCTATATT-CTCGAG-AATATAGAGTCCTCATCATTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGATGAGGACTCTATATTCTCGAGAATATAGAGTCCTCATCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGATGAGGACTCTATATTCTCGAGAATATAGAGTCCTCATCATTC-3'

Other clones with same target sequence:

(none)