Transcript: Mouse NM_025783.3

Mus musculus charged multivesicular body protein 3 (Chmp3), mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Chmp3 (66700)
Length:
2592
CDS:
136..810

Additional Resources:

NCBI RefSeq record:
NM_025783.3
NBCI Gene record:
Chmp3 (66700)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025783.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000382428 GAATGATGAGGACTCTATATT pLKO_005 1107 3UTR 100% 15.000 21.000 N Chmp3 n/a
2 TRCN0000098066 CCTCCCAAAGAGCTGGTTAAT pLKO.1 166 CDS 100% 13.200 18.480 N Chmp3 n/a
3 TRCN0000318281 CCTCCCAAAGAGCTGGTTAAT pLKO_005 166 CDS 100% 13.200 18.480 N Chmp3 n/a
4 TRCN0000098065 CGCCTGGATTTCTTCGTTGTT pLKO.1 2389 3UTR 100% 4.950 6.930 N Chmp3 n/a
5 TRCN0000381798 CACGATCTGCAGAGGATTTAA pLKO_005 958 3UTR 100% 15.000 12.000 N Chmp3 n/a
6 TRCN0000098069 TGCCTCCAAAGCACACATGAA pLKO.1 369 CDS 100% 4.950 3.960 N Chmp3 n/a
7 TRCN0000318201 TGCCTCCAAAGCACACATGAA pLKO_005 369 CDS 100% 4.950 3.960 N Chmp3 n/a
8 TRCN0000382215 GAGTTGTTGACAGGCAAATAA pLKO_005 218 CDS 100% 15.000 10.500 N CHMP3 n/a
9 TRCN0000098067 TCTTGTGAAGATCCCAGAAAT pLKO.1 483 CDS 100% 13.200 9.240 N Chmp3 n/a
10 TRCN0000318284 TCTTGTGAAGATCCCAGAAAT pLKO_005 483 CDS 100% 13.200 9.240 N Chmp3 n/a
11 TRCN0000098068 CCAAGGAGATGATCAGGTCAA pLKO.1 326 CDS 100% 4.050 2.835 N Chmp3 n/a
12 TRCN0000318282 CCAAGGAGATGATCAGGTCAA pLKO_005 326 CDS 100% 4.050 2.835 N Chmp3 n/a
13 TRCN0000141788 GAAGAGGGAGAAGAGGAAGAA pLKO.1 736 CDS 100% 4.950 2.475 Y FAM9B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025783.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03356 pDONR223 100% 89.2% 95.9% None (many diffs) n/a
2 ccsbBroad304_03356 pLX_304 0% 89.2% 95.9% V5 (many diffs) n/a
3 TRCN0000466056 GATTGTGGGACCCCCCCAGGACAG pLX_317 56.5% 89.2% 95.9% V5 (many diffs) n/a
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