Construct: shRNA TRCN0000412520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178655.3-1615s21c1
DNA Barcode:
ATTATCTGTGTGCCATATATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ank2 (109676)
Gene Description:
ankyrin 2, brain
Transcript:
RefSeq NM_178655.3 (CURRENT)
Match location:
Position 1615 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTATCTGTGTGCCATATATG
Hairpin Sequence:
5'-CCGG-ATTATCTGTGTGCCATATATG-CTCGAG-CATATATGGCACACAGATAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTATCTGTGTGCCATATATGCTCGAGCATATATGGCACACAGATAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTATCTGTGTGCCATATATGCTCGAGCATATATGGCACACAGATAAT-3'

Other clones with same target sequence:

(none)