Construct: shRNA TRCN0000412580

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008093.2-1861s21c1
DNA Barcode:
CATACTCCAGGCAGGCAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gata5 (14464)
Gene Description:
GATA binding protein 5
Transcript:
RefSeq NM_008093.2 (CURRENT)
Match location:
Position 1861 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412580)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATACTCCAGGCAGGCAATAA
Hairpin Sequence:
5'-CCGG-CATACTCCAGGCAGGCAATAA-CTCGAG-TTATTGCCTGCCTGGAGTATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATACTCCAGGCAGGCAATAACTCGAGTTATTGCCTGCCTGGAGTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATACTCCAGGCAGGCAATAACTCGAGTTATTGCCTGCCTGGAGTATG-3'

Other clones with same target sequence:

(none)