Construct: shRNA TRCN0000412925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025382.6-2355s21c1
DNA Barcode:
ATAACATCCAAGTCTGATTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem57 (66146)
Gene Description:
transmembrane protein 57
Transcript:
RefSeq NM_025382.6 (CURRENT)
Match location:
Position 2355 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATAACATCCAAGTCTGATTAG
Hairpin Sequence:
5'-CCGG-ATAACATCCAAGTCTGATTAG-CTCGAG-CTAATCAGACTTGGATGTTAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATAACATCCAAGTCTGATTAGCTCGAGCTAATCAGACTTGGATGTTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATAACATCCAAGTCTGATTAGCTCGAGCTAATCAGACTTGGATGTTAT-3'

Other clones with same target sequence:

(none)