Construct: shRNA TRCN0000413103

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016889.3-1930s21c1
DNA Barcode:
ACTCCCATTCCAGGGTGAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Insm1 (53626)
Gene Description:
insulinoma-associated 1
Transcript:
RefSeq NM_016889.3 (CURRENT)
Match location:
Position 1930 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413103)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCCCATTCCAGGGTGAAAG
Hairpin Sequence:
5'-CCGG-ACTCCCATTCCAGGGTGAAAG-CTCGAG-CTTTCACCCTGGAATGGGAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCCCATTCCAGGGTGAAAGCTCGAGCTTTCACCCTGGAATGGGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCCCATTCCAGGGTGAAAGCTCGAGCTTTCACCCTGGAATGGGAGT-3'

Other clones with same target sequence:

(none)