Construct: shRNA TRCN0000413156

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012296.3-1493s21c1
DNA Barcode:
GCCCATCACTTTGACTCACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAB2 (9846)
Gene Description:
GRB2 associated binding protein 2
Transcript:
RefSeq NM_012296.3 (CURRENT)
Match location:
Position 1493 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413156)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATCACTTTGACTCACTT
Hairpin Sequence:
5'-CCGG-GCCCATCACTTTGACTCACTT-CTCGAG-AAGTGAGTCAAAGTGATGGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATCACTTTGACTCACTTCTCGAGAAGTGAGTCAAAGTGATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATCACTTTGACTCACTTCTCGAGAAGTGAGTCAAAGTGATGGGC-3'

Other clones with same target sequence:

(none)