Construct: shRNA TRCN0000413699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002588.2-2043s21c1
DNA Barcode:
CTCTCACGGTCTTGATCAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCDHGC3 (5098)
Gene Description:
protocadherin gamma subfamily C, 3
Transcript:
RefSeq NM_002588.2 (NON-CURRENT)
Match location:
Position 2043 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTCACGGTCTTGATCAAAG
Hairpin Sequence:
5'-CCGG-CTCTCACGGTCTTGATCAAAG-CTCGAG-CTTTGATCAAGACCGTGAGAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTCACGGTCTTGATCAAAGCTCGAGCTTTGATCAAGACCGTGAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTCACGGTCTTGATCAAAGCTCGAGCTTTGATCAAGACCGTGAGAG-3'

Other clones with same target sequence:

(none)