Construct: shRNA TRCN0000414451

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019912.2-1307s21c1
DNA Barcode:
ACGTGAAGCTGTTTCTATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ube2d2a (56550)
Gene Description:
ubiquitin-conjugating enzyme E2D 2A
Transcript:
RefSeq NM_019912.2 (CURRENT)
Match location:
Position 1307 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414451)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTGAAGCTGTTTCTATATT
Hairpin Sequence:
5'-CCGG-ACGTGAAGCTGTTTCTATATT-CTCGAG-AATATAGAAACAGCTTCACGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTGAAGCTGTTTCTATATTCTCGAGAATATAGAAACAGCTTCACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTGAAGCTGTTTCTATATTCTCGAGAATATAGAAACAGCTTCACGT-3'

Other clones with same target sequence:

(none)