Construct: shRNA TRCN0000414495

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177092.4-926s21c1
DNA Barcode:
AGTTCTGCCCATCCATGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Msrb3 (320183)
Gene Description:
methionine sulfoxide reductase B3
Transcript:
RefSeq NM_177092.4 (CURRENT)
Match location:
Position 926 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414495)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTCTGCCCATCCATGTATT
Hairpin Sequence:
5'-CCGG-AGTTCTGCCCATCCATGTATT-CTCGAG-AATACATGGATGGGCAGAACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTCTGCCCATCCATGTATTCTCGAGAATACATGGATGGGCAGAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTCTGCCCATCCATGTATTCTCGAGAATACATGGATGGGCAGAACT-3'

Other clones with same target sequence:

(none)