Transcript: Mouse NM_177092.4

Mus musculus methionine sulfoxide reductase B3 (Msrb3), mRNA.

Source:
NCBI, updated 2017-06-25
Taxon:
Mus musculus (mouse)
Gene:
Msrb3 (320183)
Length:
3723
CDS:
181..741

Additional Resources:

NCBI RefSeq record:
NM_177092.4
NBCI Gene record:
Msrb3 (320183)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_177092.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000414057 GAAGCTATAAGGGCAGTTTAT pLKO_005 835 3UTR 100% 13.200 18.480 N Msrb3 n/a
2 TRCN0000085618 CTCAGGGTCATGTAGGGATAA pLKO.1 252 CDS 100% 10.800 15.120 N Msrb3 n/a
3 TRCN0000085622 GATACTGCATCAACTCAGCAT pLKO.1 626 CDS 100% 2.640 3.696 N Msrb3 n/a
4 TRCN0000085621 GTACCATGTCACTCAGGAGAA pLKO.1 333 CDS 100% 0.000 0.000 N Msrb3 n/a
5 TRCN0000429388 CACAAAGATCCTGGAATATAT pLKO_005 391 CDS 100% 15.000 10.500 N Msrb3 n/a
6 TRCN0000414495 AGTTCTGCCCATCCATGTATT pLKO_005 926 3UTR 100% 13.200 9.240 N Msrb3 n/a
7 TRCN0000419918 CAGAAGGAAGCGGCATCAAAG pLKO_005 680 CDS 100% 10.800 7.560 N Msrb3 n/a
8 TRCN0000426589 TAGATTGCTAGGCTGCGATAA pLKO_005 1219 3UTR 100% 10.800 7.560 N Msrb3 n/a
9 TRCN0000085620 ACTCCGTTGTTCAAGTCAGAA pLKO.1 430 CDS 100% 4.950 3.465 N Msrb3 n/a
10 TRCN0000085619 CATCGAGTTCACAGATGACTT pLKO.1 510 CDS 100% 4.950 3.465 N Msrb3 n/a
11 TRCN0000412559 CCTTTGAAGGAGAGTACACAC pLKO_005 368 CDS 100% 4.050 2.835 N Msrb3 n/a
12 TRCN0000166364 CACACACACACACACACACAA pLKO.1 2819 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_177092.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05292 pDONR223 100% 85.8% 92% None (many diffs) n/a
2 ccsbBroad304_05292 pLX_304 0% 85.8% 92% V5 (many diffs) n/a
3 TRCN0000472383 AGTTTACATGTATCCGCCAGTTAC pLX_317 85.9% 85.8% 92% V5 (many diffs) n/a
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