Construct: shRNA TRCN0000415511

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175533.3-984s21c1
DNA Barcode:
ACAGAGGCCTTTCGCTGTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
5830411N06Rik (244234)
Gene Description:
RIKEN cDNA 5830411N06 gene
Transcript:
RefSeq NM_175533.3 (CURRENT)
Match location:
Position 984 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415511)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGAGGCCTTTCGCTGTATG
Hairpin Sequence:
5'-CCGG-ACAGAGGCCTTTCGCTGTATG-CTCGAG-CATACAGCGAAAGGCCTCTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGAGGCCTTTCGCTGTATGCTCGAGCATACAGCGAAAGGCCTCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGAGGCCTTTCGCTGTATGCTCGAGCATACAGCGAAAGGCCTCTGT-3'

Other clones with same target sequence:

(none)