Construct: shRNA TRCN0000415516

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002471.3-239s21c1
DNA Barcode:
AGGCAAGGTCATTGCTGAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYH6 (4624)
Gene Description:
myosin heavy chain 6
Transcript:
RefSeq NM_002471.3 (CURRENT)
Match location:
Position 239 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415516)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCAAGGTCATTGCTGAAAC
Hairpin Sequence:
5'-CCGG-AGGCAAGGTCATTGCTGAAAC-CTCGAG-GTTTCAGCAATGACCTTGCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCAAGGTCATTGCTGAAACCTCGAGGTTTCAGCAATGACCTTGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCAAGGTCATTGCTGAAACCTCGAGGTTTCAGCAATGACCTTGCCT-3'

Other clones with same target sequence:

(none)