Construct: shRNA TRCN0000415758

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199304.1-2538s21c1
DNA Barcode:
CAGAGTAACCAGGCTAGACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp341 (228807)
Gene Description:
zinc finger protein 341
Transcript:
RefSeq NM_199304.1 (CURRENT)
Match location:
Position 2538 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415758)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGTAACCAGGCTAGACTT
Hairpin Sequence:
5'-CCGG-CAGAGTAACCAGGCTAGACTT-CTCGAG-AAGTCTAGCCTGGTTACTCTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGTAACCAGGCTAGACTTCTCGAGAAGTCTAGCCTGGTTACTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGTAACCAGGCTAGACTTCTCGAGAAGTCTAGCCTGGTTACTCTG-3'

Other clones with same target sequence:

(none)