Construct: shRNA TRCN0000415788

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138652.2-650s21c1
DNA Barcode:
TACTTGTCCTGGTCGTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp12a (192113)
Gene Description:
ATPase, H+/K+ transporting, nongastric, alpha polypeptide
Transcript:
RefSeq NM_138652.2 (CURRENT)
Match location:
Position 650 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415788)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACTTGTCCTGGTCGTTATTT
Hairpin Sequence:
5'-CCGG-TACTTGTCCTGGTCGTTATTT-CTCGAG-AAATAACGACCAGGACAAGTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACTTGTCCTGGTCGTTATTTCTCGAGAAATAACGACCAGGACAAGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACTTGTCCTGGTCGTTATTTCTCGAGAAATAACGACCAGGACAAGTA-3'

Other clones with same target sequence:

(none)