Transcript: Mouse NM_138652.2

Mus musculus ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Atp12a (192113)
Length:
3954
CDS:
214..3321

Additional Resources:

NCBI RefSeq record:
NM_138652.2
NBCI Gene record:
Atp12a (192113)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_138652.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000101624 GCCATCATGGTCCAACAAATT pLKO.1 3019 CDS 100% 13.200 10.560 N Atp12a n/a
2 TRCN0000101622 CCTCGTCTATTTCACTGTCTA pLKO.1 2844 CDS 100% 4.950 3.960 N Atp12a n/a
3 TRCN0000435658 TAAAGTTCTCAGAGGTTATTT pLKO_005 1616 CDS 100% 15.000 10.500 N Atp12a n/a
4 TRCN0000415788 TACTTGTCCTGGTCGTTATTT pLKO_005 650 CDS 100% 15.000 10.500 N Atp12a n/a
5 TRCN0000425688 GATGACAACTTTGCATCTATA pLKO_005 2500 CDS 100% 13.200 9.240 N Atp12a n/a
6 TRCN0000101623 GCCAAGAGTGTAGGAATCATA pLKO.1 2128 CDS 100% 5.625 3.938 N Atp12a n/a
7 TRCN0000101621 CCAAGATAATAACACTGTGTA pLKO.1 1511 CDS 100% 4.950 3.465 N Atp12a n/a
8 TRCN0000101620 GCCTTAAGCTAGGCTGTGATT pLKO.1 3651 3UTR 100% 4.950 3.465 N Atp12a n/a
9 TRCN0000421890 GTGACTTAAGTGCTAATATTT pLKO_005 3777 3UTR 100% 15.000 9.000 N Atp12a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_138652.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05866 pDONR223 100% 84.5% 86.1% None (many diffs) n/a
2 ccsbBroad304_05866 pLX_304 0% 84.5% 86.1% V5 (not translated due to frame shift) (many diffs) n/a
3 TRCN0000467164 GACTCTCCTCGTACCATCCACGCC pLX_317 13.5% 84.5% 86.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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