Construct: shRNA TRCN0000415827

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175750.3-4369s21c1
DNA Barcode:
AGACCAACTTCACCTATTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plxna4 (243743)
Gene Description:
plexin A4
Transcript:
RefSeq NM_175750.3 (CURRENT)
Match location:
Position 4369 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415827)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACCAACTTCACCTATTATC
Hairpin Sequence:
5'-CCGG-AGACCAACTTCACCTATTATC-CTCGAG-GATAATAGGTGAAGTTGGTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACCAACTTCACCTATTATCCTCGAGGATAATAGGTGAAGTTGGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACCAACTTCACCTATTATCCTCGAGGATAATAGGTGAAGTTGGTCT-3'

Other clones with same target sequence:

(none)