Construct: shRNA TRCN0000416159

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133374.2-2595s21c1
DNA Barcode:
GTGTGAACAAGCGCTTCTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF618 (114991)
Gene Description:
zinc finger protein 618
Transcript:
RefSeq NM_133374.2 (CURRENT)
Match location:
Position 2595 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416159)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTGAACAAGCGCTTCTAAT
Hairpin Sequence:
5'-CCCG-GTGTGAACAAGCGCTTCTAAT-CTCGAG-ATTAGAAGCGCTTGTTCACAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTGAACAAGCGCTTCTAATCTCGAGATTAGAAGCGCTTGTTCACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTGAACAAGCGCTTCTAATCTCGAGATTAGAAGCGCTTGTTCACAC-3'

Other clones with same target sequence:

(none)